By Hanne Blank
Just like the typewriter and the sunshine bulb, the heterosexual used to be invented within the 1860s and quickly and completely reworked Western tradition. the belief of “the heterosexual” was once remarkable. in any case, women and men have been having intercourse, marrying, development households, and occasionally even falling in love for millennia with no need any unique identify for his or her feelings or acts. but, inside of part a century, “heterosexual” had turn into a byword for “normal,” enshrined in legislations, medication, psychiatry, and the media as a brand new optimum for human experience.
during this astonishing chronicle, historian Hanne clean digs deep into the previous of sexual orientation, whereas concurrently exploring its modern psyche. Illuminating the hidden styles in centuries of occasions and traits, clean indicates how tradition creates and manipulates the methods we predict approximately and adventure hope, love, and relationships among women and men. starting from Henry VIII to testicle transplants, from Disneyland to sodomy legislation, and from Moby Dick to man made insemination, the heritage of heterosexuality seems to be something yet immediately or narrow.
With an eclectic scope and interesting aspect, Straight tells the eye-opening tale of a fancy and infrequently contradictory man-made construction that's all too usually assumed to be an irreducible truth of biology.
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Additional info for Straight: The Surprisingly Short History of Heterosexuality
The homogenate) to ultracentrifugation tubes and obtain membrane fractions by ultracentrifugation (60 min at 100,000 × g and 4 °C) (see Note 35). 7. , the membrane fraction) on ice in lysis buffer (see Note 36). 8. 0 mg/ml) as standard. 9. 35 mg/ml using lysis buffer, glycerol, and 10 % (w/v) digitonin to reach a final concentration of 10 % (v/v) glycerol and 1 % (w/v) digitonin (see Note 37). When performing AP-PM experiments, continue with equal total protein amounts obtained from POI-tagged and control cells.
14. Protease inhibitors may inhibit TEV protease as well as tryptic protein digest. , phosphatase inhibitors) to the elution buffer is required, make sure they do not impair the activity of the TEV protease and trypsin. 15. , from GE Healthcare) or can be prepared as described . 16. For sample preparation and subsequent LC-MS analysis, use only HPLC grade solvents and reagents as well as water of Milli-Q purity. 17. The protocol described in this chapter including the analysis of mass spectrometric raw data relies on the acquisition of high resolution MS data and the use of Orbitrap instruments.
Slices (fractions) may also be designated if further fractionation methodologies were performed (examples include off gel fractionation or in gel fractionation). 29. The number of threads may be set according to the number of RAW files. However, the total number of threads should not exceed the total capacity of the operating computer (number of cores). 5 GB of RAM is required. For optimal processing performance, at least twice the size of the total size of all RAW files being analyzed should be available on the hard disk space.