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By Abelson J.N., Simon M.I., Phillips I.M.

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Saruta, and J. Miyazaki, Biochem. Biophys. Res. Commun. 233(2), 527 (1997). 8 H. Aihara and J. Miyazaki, Nature Biotechnol. 16(9), 867 (1998). 9 A. Decrouy, J. M. Renaud, J. A. Lunde, G. Dickson, and B. J. Jasmin, Gene Ther. 5(1), 59 (1998). 10H. Alila, M. Coleman, H. Nitta, M. French, K. Anwer, Q. Liu, T. Meyer, J. Wang, R. Mumper, D. Oubari, S. Long, J. Nordstrom, and A. Rolland, Hum. Gene Ther. 8(15), 1785 (1997). 11 y. Tsurumi, M. Kearney, D. Chen, M. Silver, S. Takeshita, J. Yang, J. F. Symes, and J.

Antisense technology represents a rational approach to address this important issue. [3] Delivery of Antisense DNA by Vectors for Prolonged Effects in Vitro a n d in Vivo By DAGMARAMOHUCZY, XIAOPING TANG, and M. IAN PHILLIPS Introduction Vectors (plasmid or viruses) can be used for delivery of the gene of interest in "sense" or "antisense" orientation. The sense orientation is to express, as a final product, active protein capable of performing physiological function. The antisense orientation is to produce antisense mRNA that interferes with normally produced "sense" mRNA and, as a result, decrease the amount of translated protein.

O'Malley, Proc. Natl. Acad. Sci. A. 96(2), 355 (1999). 43 S. Kochanek, P. R. Clemens, K. Mitani, H. H. Chen, S. Chan, and C. T. Caskey, Proc. Natl. Acad. Sci. A. 93(12), 5731 (1996). 44 It has been used successfully for delivering antisense R N A against a-globin 45 and human immunodeficiency virus (HIV) long terminal repeats, 46 and it is our vector of choice for delivering antisense targeted to the AT1 receptor in cell culture and hypertensive rat models. A A V is a parvovirus, discovered as a contamination of adenoviral stocks.

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