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By Saul Neidleman

"Advances in utilized Microbiology" covers a extensive variety of themes within the fields of utilized microbiology and biotechnology. those volumes supply articles which might be of curiosity to biotechnology researchers in academia and undefined, fermentation microbiologists, microbial ecologists, biochemical engineers and utilized microbiologists in different speciality components.

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5 liters of tap water for 2 hours: cool overnight, filter (Whatman No. 2), dilute to 5 liters with tap water, autoclave for 15 min at 121OC. and store at 4°C. 03 or 1 ml, respectively, of at least four serial soil dilutions to five replicate wells or tubes. Incubate the tubes on a slant, and do not screw the caps on tightly, to enhance aeration of the medium. Incubate for at least 7 days at 24 & 2°C. , flagellates, ciliates, amoebas) separately. 1-ml aliquots from the test tubes under lowpower magnification ( x 100) for the presence of protozoa.

Zero the spectrophotometer with a methanol blank. Convert the data to an ovendry basis by multiplying the TPF values by 1plus the percent soil water content (expressed as a decimal). Record the data as micrograms TPF per gram soil, oven-dry equivalent. , 1990). , species diversity, antibiograms, biochemical and nutritional characteristics, enzyme activity, fate of the GEMs), both over time and between uninoculated soil and the soil inoculated with a GEM or the homologous parental strain. , CO, evolution and dehydrogenase activity) provides an internal control on the validity and sensitivity of the individual indicators.

1990). , species diversity, antibiograms, biochemical and nutritional characteristics, enzyme activity, fate of the GEMs), both over time and between uninoculated soil and the soil inoculated with a GEM or the homologous parental strain. , CO, evolution and dehydrogenase activity) provides an internal control on the validity and sensitivity of the individual indicators. Furthermore, the degree of correlation between the various indicators should identify those assays that are clearly redundant and can be eliminated in the further development of a standard battery of assays with which to evaluate the potential impacts of any GEMs introduced into soil or other natural habitats on microbial populations and microbe-mediated ecological processes.

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